منابع مشابه
ImageJ for microscopy.
ImageJ is an essential tool for us that fulfills most of our routine image processing and analysis requirements. The near-comprehensive range of import filters that allow easy access to image and meta-data, a broad suite processing and analysis routine, and enthusiastic support from a friendly mailing list are invaluable for all microscopy labs and facilities-not just those on a budget.
متن کامل3D PSF Models for Fluorescence Microscopy in ImageJ
The current version allows for five different PSF models: The Gaussian model simulates a blurring effect by setting three different variance values. These values characterize the width of the PSF at various axial positions along the z-stack. The defocus model is simulated in the Fourier domain by a modulated Gaussian where the sinc modulation depends on the axial defocusing distance. Another de...
متن کاملQSIM: quantitative structured illumination microscopy image processing in ImageJ
BACKGROUND Structured illumination microscopy has been extensively used in biological imaging due to its low cost and easy implementation. However, the lack of quantitative imaging capability limits its application in absolute irradiance measurements. METHOD We have developed a quantitative structured illumination microscopy image processing algorithm (QSIM) as a plugin for the widely used Im...
متن کاملA simple viability analysis for unicellular cyanobacteria using a new autofluorescence assay, automated microscopy, and ImageJ
BACKGROUND Currently established methods to identify viable and non-viable cells of cyanobacteria are either time-consuming (eg. plating) or preparation-intensive (eg. fluorescent staining). In this paper we present a new and fast viability assay for unicellular cyanobacteria, which uses red chlorophyll fluorescence and an unspecific green autofluorescence for the differentiation of viable and ...
متن کاملSMLocalizer, a GPU accelerated ImageJ plugin for single molecule localization microscopy
Summary SMLocalizer combines the availability of ImageJ with the power of GPU processing for fast and accurate analysis of single molecule localization microscopy data. Analysis of 2D and 3D data in multiple channels is supported. Availability and implementation Plugin freely available for Fiji and ImageJ2.0 through https://sourceforge.net/projects/smlocalizer/. Plugin also available for cont...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2007
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000112517